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Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin

机译：Moesin的主要细胞特异性表达揭示了胃腺组织的新见解

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ERM (ezrin, radixin, and moesin) proteins play critical roles in epithelial and endothelial cell polarity, among other functions. In gastric glands, ezrin is mainly expressed in acid-secreting parietal cells, but not in mucous neck cells or zymogenic chief cells. In looking for other ERM proteins, moesin was found lining the lumen of much of the gastric gland, but it was not expressed in parietal cells. No significant radixin expression was detected in the gastric glands. Moesin showed an increased gradient of expression from the neck to the base of the glands. In addition, the staining pattern of moesin revealed a branched morphology for the gastric lumen. This pattern of short branches extending from the glandular lumen was confirmed by using antibody against zonula occludens-1 (ZO-1) to stain tight junctions. With a mucous neck cell probe (lectin GSII, from Griffonia simplicifolia) and a chief cell marker (pepsinogen C), immunohistochemistry revealed that the mucous neck cells at the top of the glands do not express moesin, but, progressing toward the base, mucous cells showing decreased GSII staining had low or moderate level of moesin expression. The level of moesin expression continued to increase toward the base of the glands and reached a plateau in the base where chief cells and parietal cells abound. The level of pepsinogen expression also increased toward the base. Pepsinogen C was located on cytoplasmic granules and/or more generally distributed in chief cells, whereas moesin was exclusively expressed on the apical membrane. This is a clear demonstration of distinctive cellular expression of two ERM family members in the same tissue. The results provide the first evidence that moesin is involved in the cell biology of chief cells. Novel insights on gastric gland morphology revealed by the moesin and ZO-1 staining provide the basis for a model of cell maturation and migration within the gland.
机译：ERM（ezrin，Radixin和Moesin）蛋白在其他功能中，在上皮和内皮细胞极性中起关键作用。在胃腺中，ezrin主要在分泌酸的顶细胞中表达，但在粘液颈部细胞或酶原性主细胞中不表达。在寻找其他ERM蛋白时，发现在大部分胃腺腔内衬有肌动蛋白，但在壁细胞中未表达。在胃腺中未检测到明显的放射蛋白表达。 Moesin显示从颈部到腺体的表达梯度增加。另外，肌红蛋白的染色模式揭示了胃腔的分支形态。通过使用对小带闭合的抗体（ZO-1）染色紧密连接，可以确认从腺腔延伸出的短分支模式。免疫组织化学显示，使用粘液颈细胞探针（凝集素GSII，来自格里弗尼亚州辛普里弗利娅）和主要细胞标记物（胃蛋白酶原C）后，腺体顶部的粘液颈细胞不表达肌动蛋白，而是向基底，粘液方向发展GSII染色减少的细胞中Moesin表达水平较低或中等。肌动蛋白的表达水平朝着腺体的底部继续增加，并在底部达到平台，在那里主要细胞和顶壁细胞比比皆是。胃蛋白酶原的表达水平也朝着碱基增加。胃蛋白酶原C位于细胞质颗粒上和/或更普遍地分布在主细胞中，而肌球蛋白仅在顶膜上表达。这清楚地证明了两个ERM家族成员在同一组织中的独特细胞表达。该结果提供了第一个证据，证明moesin参与了主细胞的细胞生物学。 Moesin和ZO-1染色揭示的关于胃腺形态的新颖见解为腺内细胞成熟和迁移的模型提供了基础。

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